The Three-Species Consortium of Genetically Improved Strains Cupriavidus necator RW112, Burkholderia xenovorans RW118, and Pseudomonas pseudoalcaligenes RW120 Grows with Technical Polychlorobiphenyl, Aroclor 1242

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The Three-Species Consortium of Genetically Improved Strains Cupriavidus necator RW112, Burkholderia xenovorans RW118, and Pseudomonas pseudoalcaligenes RW120 Grows with Technical Polychlorobiphenyl, Aroclor 1242

Burkholderia xenovorans LB400, Cupriavidus necator H850, and Pseudomonas pseudoalcaligenes KF707 are bacterial strains able to mineralize biphenyl and to co-oxidize many of its halogenated derivatives (PCBs). Only strain LB400 also mineralizes a few mono- and dichlorobiphenyls, due to the presence of a functioning chlorocatechol pathway. Here, we used a Tn5-based minitransposon shuttle system t...

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Growth of the genetically engineered strain Cupriavidus necator RW112 with chlorobenzoates and technical chlorobiphenyls.

Cupriavidus necator (formerly Ralstonia eutropha) strain H850 is known to grow on biphenyl, and to co-oxidize congeners of polychlorinated biphenyls (PCBs). Using a Tn5-based minitransposon shuttle system and the TOL plasmid, the rational construction of hybrids of H850 was achieved by subsequent introduction of three distinct elements carrying 11 catabolic loci from three other biodegrading ba...

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Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

Renewable resources constitute a ‘limited infinity’, meaning that a limited amount is being produced, but for an infinite time.1 One of the most promising approaches to reduce the resource intensity is to use the existing biomass more efficiently: the biorefinery concept. It aims to use the complete biomass and all by-products emitted by the processes. Particularly the by-products of establishe...

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Evaluation of Degradability of Aroclor 1242 by an Aceticlastic Methanogenic Consortium

The rate and extent of degradation of Aroclor 1242 from contaminated surface soil was investigated, using aceticlastic mathanogenic consortia enriched from municipal anaerobic digester. Two different experiments, “intermediate feed” and “starve and feed” were conducted using triplicate 160-mL serum bottle microcosms, containing either 0.1 g, 1 g, 10 g, or 50 g of soil plus 100 mL of the inoculu...

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Aroclor 1242 inhalation and ingestion by Sprague-Dawley rats.

PCBs have been considered to be almost nonvolatile and insoluble in water. However, recent studies have shown the importance of their slight solubility in water and capability to enter the atmosphere and disperse throughout the global environment. This preliminary study was designed to measure uptake and observe any physiological changes in Sprague-Dawley rats. The PCB product Aroclor 1242 is t...

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ژورنال

عنوان ژورنال: Frontiers in Microbiology

سال: 2013

ISSN: 1664-302X

DOI: 10.3389/fmicb.2013.00090